Cloned (Comment) | Organism |
---|---|
gene PLAT, recombinant human tPA (residue 297-562) is expressed in Expi293 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | screening of a peptide aldehyde library against tPA, inhibitory potencies of compounds at 0.01 mM, analysis of effects of different amino acids on positions P4, P3, P2, and P1, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
0.48 | - |
acetyl-FTAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
0.59 | - |
acetyl-FYAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
0.76 | - |
acetyl-GTGR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
1.1 | - |
acetyl-GTAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
1.3 | - |
acetyl-QYAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00750 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-FTAR-4-nitroanilide + H2O | - |
Homo sapiens | acetyl-FTAR + 4-nitroaniline | - |
? | |
acetyl-FYAR-4-nitroanilide + H2O | - |
Homo sapiens | acetyl-FYAR + 4-nitroaniline | - |
? | |
acetyl-GTAR-4-nitroanilide + H2O | - |
Homo sapiens | acetyl-GTAR + 4-nitroaniline | - |
? | |
acetyl-GTGR-4-nitroanilide + H2O | - |
Homo sapiens | acetyl-GTGR + 4-nitroaniline | - |
? | |
acetyl-QYAR-4-nitroanilide + H2O | - |
Homo sapiens | acetyl-QYAR + 4-nitroaniline | - |
? | |
benzyl-TAR-4-nitroanilide + H2O | - |
Homo sapiens | benzyl-TAR + 4-nitroaniline | - |
? | |
additional information | substrate specificity of enzyme tissue-type plasminogen activator, comparison with urokinase-type plasminogen activator, EC 3.4.21.73. The urokinase-type plasminogen activator shows much higher activity towards the peptide substrates | Homo sapiens | ? | - |
? | |
Z-Pyr-Gly-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Z-Pyr-Gly-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PLAT | - |
Homo sapiens |
Tissue-type plasminogen activator | - |
Homo sapiens |
tPA | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
acetyl-GTGR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
0.24 | - |
acetyl-FTAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
0.36 | - |
acetyl-GTAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
0.46 | - |
acetyl-QYAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
0.86 | - |
acetyl-FYAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) are two serine proteases that contribute to initiating fibrinolysis by activating plasminogen. uPA is also an important tumour-associated protease due to its role in extracellular matrix remodelling | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
acetyl-GTGR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
0.33 | - |
acetyl-GTAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
0.35 | - |
acetyl-QYAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
0.5 | - |
acetyl-FTAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens | |
1.46 | - |
acetyl-FYAR-4-nitroanilide | pH and temperature not specified in the publication | Homo sapiens |